Effect of induction therapy with bone mesenchymal stem cells on adenosine triphosphate levels in CD4+ T cells determined by ImmuKnow assay in patients receiving renal transplantation

doi:10.3969/j.issn.2095-4344.2014.37.012

Abstract

Abstract:

BACKGROUND: Bone mesenchymal stem cells have immunological regulation function both in vitro and in vivo, while the effect of bone marrow mesenchymal stem cells on CD4⁺ T cell immune function in patients receiving kidney transplantation remains unclear.
OBJECTIVE: To explore the monitoring significance of CD4⁺ T-cell immune function by ImmuKnow assay and to determine the effect of induction therapy with bone marrow mesenchymal stem cells on cell immune function in patients receiving kidney transplantation.
METHODS: From January 2011 to June 2013, 24 patients receiving allograft renal transplantation with autologous bone marrow mesenchymal stem cells were included and another 48 patients receiving allograft renal transplantation and Simulect induction therapy with various matched preoperative characters served as controls. In both groups, adenosine triphosphate levels in CD4⁺ T cells in the peripheral blood were determined by the ImmuKnow assay preoperatively and at 14, 30, 60, 90, 180 days postoperatively, as well as during acute rejection and infection episodes.
RESULTS AND CONCLUSION: During the 180 days postoperatively, fewer patients in the bone marrow mesenchymal stem cell group had acute rejection and injection than the Simulect group, but no significant differences were observed. Postoperative adenosine triphosphate levels in CD4⁺ T cells were significantly lower than those determined preoperatively in both groups (P < 0.05), while no significant differences were observed between the two groups. A total of 12 patients in the bone marrow mesenchymal stem cell group and 26 patients in the Simulect group had infection episodes, and the adenosine triphosphate levels in CD4⁺ T cells during the infection episodes were lower than clinical stable patients in both groups (P < 0.01). For patients receiving renal transplantation, induction therapy with bone marrow mesenchymal stem cells can effectively decrease the cell immune function, which can be reflected by the adenosine triphosphate levels in CD4⁺ T cells in the peripheral blood determined by the ImmuKnow assay.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, kidney transplantation, adenosine triphosphate, immunity

CLC Number:

R394.2

Chen Shu-shang, Cai Jin-quan, Wu Cheng-yao, Deng Zhen, Zhu Ling-feng, Zhou Hao, Wang Qing-hua, Tan Jian-ming . Effect of induction therapy with bone mesenchymal stem cells on adenosine triphosphate levels in CD4⁺ T cells determined by ImmuKnow assay in patients receiving renal transplantation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 5972-5976.

Figures/Tables 2

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